Kichere, Odao2021-08-272021-08-272019Kichere, Odao (2019) Isoflavonoids from the root barks of millettia schliebenii, Master dissertation, University of Dar es Salaamhttp://41.86.178.5:8080/xmlui/handle/123456789/15490Available in print form, East Africana Collection, Dr. Wilbert Chagula Library, Class mark (THS EAF QK495.L52K523)The phytochemical investigations reported in this dissertation were aimed at the isolation of biologically active and other constituents from the root barks of Millettiaschliebenii Harms (Fabaceae). The plant species belongs to Millettia, a genus of Tanzanian plants that is known for numerous therapeutic indications such as antiviral, bactericidal, anti-inflammatory, insecticidal, and pesticidal. Three isoflavonoids were isolated from the methanolic extract of the root barks of Millettiaschliebenii using chromatographic technique. Structures of the isolated compounds were identified by NMR and MS techniques as maximaisoflavone C (2.26), 7,2′-dimethoxy-4′,5′-methylenedioxyisoflavone (2.27) and durlettone (2.28). The disc diffusion method was used to test for activity of crude extract against both Gram-negative (Escherichia coli, Salmonella typhi and Klebsiella pneumonia) and Gram-positive (Staphylococcus aureus) bacteria. Isolated compounds were tested using broth micro-dillusion method against Gram negative (Escherichia coli) and Gram positive Bacillus subtilis). The crude extract and the three compounds were inactive in antibacterial assays at a concentration of 100 mg/mL for crude extract and 1 mg/mL for isolated pure compounds. Thus, the reported results from this study suggest the foundation for more investigations on antibacterial agents from other parts (leaves and stems barks) of M. schliebeniior other species in the genus Millettia. Evaluation of antitrypanosomal activities were yet to be accomplished at the collaborating laboratory up to the time of submission of this dissertation.enMollettiaRootThesis