Characterisation and analysis of Antimicrobial activity of endophytic Fungi associated with medicinal plants from selected regions of Tanzania
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Date
2020
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Publisher
University of Dar es Salaam
Abstract
This study characterized and analysed antimicrobial activity of endophytic fungi associated with 10 medicinal plants from three regions of Tanzania;Dar es salaam, Tanga, and Kilimanjaro. A total of 23 endophytic fungi isolates were isolated from different parts of medicinal plants after disinfection, different biological tests were performed. The ethyl ecetate crude extracts of endophytic fungi secondary metabolities were screened for antimicrobial activity against Gram-negative bacteria (basillis substlis and staphyloccus aureus) Gram-positive (Escherichia coli) and fungi pathogen(candida albicans)The results revealed that endophytic fungi from 9 medicinal plants exhibited antimicrobial activity against either of the tested pathogenic bacteria and fungi.Of all the studied isolates, crude extract of secondary metabolites of endophytic fungi from MOIA(Ocimum suave leaf)and SW5A(Alium sativum clove) exhibited strong antimicrobial activity with minimum inhibition (MIC)ranging from 0.125 mg/ml to 0.5mg/ml against all tested pathogenic bacteria and fungi. On the other hand, micro morphological and molecular characterization of the isolated endophytes performed from these medicinal plants ascertained them to belong to 9 main genera while dominant genus found in all corrections from three sites were Colletotrichum.The results futher revealed that diversity of endophytic fungi varied irrespective of the geographical location of the host plants.The phytochemical analysis of the secondary metabolites from endophytic fungi revealed the presence of vital constituents potential for medicinal applications namely; alkaloids,phenols,flavonoids, tannins, terpenoids, tennins, steroids, amino acids, carbohydrates and saponins. This study demonstrated that secondary metabolities of endophytic fungi from the studied medicinal plants possess important phytochemical and exhibit antimicrobial potential against the tested human pathogens which could contribute to endeavors for new therapeutic inventions
Description
Available in print form, East Africana Collection, Dr.Wilbert Chagula Library, class mark (THS EAF QK604.2.E53T34M826)
Keywords
endophytic fungi, medicinal plants, Dar es salaam, Tanga, Kilimanjaro
Citation
Mwanga,Z, N(2020)Characterisation and analysis of Antimicrobial activity of endophytic Fungi associated with medicinal plants from selected regions of Tanzania,Doctorial dissertation ,University of Dar es Salaam, Dar es Salaam