Characterization and analysis of antimicrobial activity of entophytic fungi associated with medicinal plants from selected regions of Tanzania.
| dc.contributor.author | Mwanga, Zuhura Ndoika | |
| dc.date.accessioned | 2021-11-16T12:15:45Z | |
| dc.date.available | 2021-11-16T12:15:45Z | |
| dc.date.issued | 2020 | |
| dc.description | Available in print form, East Africana collection, Dr. Wilbert Chagula Library, class mark (THS EAF QK604.2.E53T34M826) | en_US |
| dc.description.abstract | The study characterized and analyzed antimicrobial activity of endophytic fungi associated with 10 medicinal plants from three regions of Tanzania, Dar es salaam, Tanga, and Kilimanjaro. A total of 23 endophytic fungi isolates were isolated from different parts of medicinal plants after disinfection, different biological tests were performed. The ethyl acetate crude extracts of endophytic fungi secondary metabolites were screened for antimicrobial activity against Gram-negative bacteria (bassillus substiles and staphylococcus aureus), Gram-positive (Escherichia coli) and fungi pathogen (Candida albicans). The results revealed that endophytic fungi from 9 medicinal plants exhibited antimicrobias activity against either of the tested pathogenic bacteria and fungi. Of all the studied isolates, crude extract of secondary metabolites of endophytic fungi from MOIO (Ocimum suave- leaf) and SW5A (alium sativum clove) exhited strong antimicrobial activity with minimum inhibition (MIC) ranging from 0.125 mg/ml to 05 mg/ml against all tested pathogenic bacteria of fungi. On the other hand, micro morphological and molecular characterization of the isolated endophytes performed from these medical plants ascertained them to belong to 9 main genera while dominant genus found in all corrections from three sites were colletotrichum. The results further revealed that diversity of endophytic fungi varied irrespective of the geographical location of the host plants. The phytochemical analysis of the secondary metabolites from endophytic fungi revealed the presence of vital constituent’s potential for medical application namely: alkaloids, phenols, flavonoids, tannins, terpenoids, tannins, steroids, amino acids, carbohydrates and saponins. This study demonstrated that secondary metabolites of endophytic fungi from the studied medicinal plants possess important phytochemical and exhibit antimicrobial potential against the tested human pathogens which could contribute to the endeavors for new therapeutic inventions. | en_US |
| dc.identifier.citation | Mwanga, Z.N.(2020)Characterization and analysis of antimicrobial activity of entophytic fungi associated with medicinal plants from selected regions of Tanzania.Doctorial dissertation,University of Dar es Salaam, Dar es Salaam. | en_US |
| dc.identifier.uri | http://41.86.178.5:8080/xmlui/handle/123456789/16474 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Dar es salaam | en_US |
| dc.subject | Endophytic fungi | en_US |
| dc.subject | Medicinal plant | en_US |
| dc.subject | Dar es salaam | en_US |
| dc.subject | Tanga | en_US |
| dc.subject | Kilimanjaro | en_US |
| dc.title | Characterization and analysis of antimicrobial activity of entophytic fungi associated with medicinal plants from selected regions of Tanzania. | en_US |
| dc.type | Thesis | en_US |