Assessment of changes in genetic diversity of nile Tilapia, Oreochromis niloticus (Linnaeus, 1758), of lake Baringo Kenya

dc.contributor.authorYeremia, Chuhira
dc.date.accessioned2020-12-07T10:12:41Z
dc.date.available2020-12-07T10:12:41Z
dc.date.issued2015
dc.descriptionAvailable in print form, East Africana Collection, Dr.Wilbert Chagula Library, class mark (THS EAF SH167.T54Y47)en_US
dc.description.abstractThe rapid expansion of tilapia aquaculture farming in the Rift Valley Region of Kenya, has led to the transfer of different Oreochromis species from farms (ponds) to natural ecosystems such as rivers and lakes. These transfers have negatively impacted on native tilapia species of these systems through competition, hybridization and introgression which consequently has led to the dwindling in number as well as compromising the genetic integrity of the native species. In Lake Baringo, introductions of tilapia from unverified sources and unknown species has been reported in the past but has continued due to the rapid expansion of tilapia farming. The present study aimed at evaluating the current genetic diversity of Oreochromis species in Lake Baringo in order to determine the recent species introductions and effects on endemic populations of the Nile tilapia Oreochromis niloticus baringoensis . Morphological (morphometric and meristics) and molecular techniques involving mtDNA markers (Cytochrome Oxidase 1, Control region and D-loop genes) were used to identify tilapia species from the lake. The observed morphometric and meristic differences showed variations congruent with two tilapia species Oreochromis niloticus and Oreochromis spirulus. Genetic studies revealed four haplotypes based on maximum likelihood phylogenetic trees on mtDNA Cytochrome Oxidase 1 and seven haplotypes each for mtDNA Control region and D-loop marker genes. Blasting, alignment and phylogenetic analysis of mtDNA D-loop partial fish sample sequences with the NCBI data base sequences for most cultured tilapia species enabled the identification of the haplotypes as 0. n. baringoensis, 0. spilurus, 0. n. vulcani, 0. niloticus (unknown) and the hybrids of 0. n. baringoensis with 0. leucostictus. These findings confirm indications that increased aquaculture activities around Lake Baringo basin have led to continued species introduction and hybridization in the main lake, thus endangering the native species 0.n baringoensis which form an important role in commercial fisheries for the communities around the lake.en_US
dc.identifier.citationYeremia, C (2015) Assessment of changes in genetic diversity of nile Tilapia, Oreochromis niloticus (Linnaeus, 1758), of lake Baringo Kenya, Masters dissertation, University of Dar es Salaam, Dar es Salaam.en_US
dc.identifier.urihttp://41.86.178.5:8080/xmlui/handle/123456789/13684
dc.language.isoenen_US
dc.publisherUniversity of Dar es Salaamen_US
dc.subjectNile Tilapiaen_US
dc.subjectTilapiaen_US
dc.subjectGermplasm Resourcesen_US
dc.subjectBaringoen_US
dc.subjectLakeen_US
dc.subjectKenyaen_US
dc.titleAssessment of changes in genetic diversity of nile Tilapia, Oreochromis niloticus (Linnaeus, 1758), of lake Baringo Kenyaen_US
dc.typeThesisen_US

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