A new pterocarpan and other constituents from erythrina schliebenii and cleistochlamys kirkii: anti-tuberculosis and anti-cancer evaluation
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Date
2014
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University of Dar es Salaam
Abstract
This dissertation reports the results of a phytochemical investigation of secondary metabolites from Erythrina schliebenii and Cleistochlamys kirkii. The dichloromethane extract of root bark of E. schliebenii was screened for antimycobacterial and anticancer properties against Mycobacterium tuberculosis (H37Rv) and human breast cancer cells (MCF-7) and upon chromatographic separation yielded a new prenylated pterocarpan 9-hydroxy-3-methoxy-2prenylpterocarpan (2.1) together with a known flavanone 4′,7-dihydroxy-3′,5′diprenylflavanone (2.2), a prenylated pterocarpan eurautenol (2.3) and an isoflavone 5′-(3-methylbut-2-enyl)pratensein (2.4). The ethanol root bark extract of C. kirkii was screened for anticancer activity and upon chromatographic separation yielded polycarpol (3.1) and cleistenolide (3.2) as well as other compounds whose structures have not been elucidated due to lack of some spectroscopic data. All isolated compounds from E. schliebenii that were tested displayed anti-tubercular activity at minimum inhibitory concentration values between 64 and 32 μg/mL while C. kirkii isolates were not assayed for this activity. Compounds 2.2 and 3.2 exhibited potent activity against human breast cancer cells at LD50 of 16.81 μg/mL and 14.78 μg/mL, respectively. Compounds 2.4 and 3.1 showed lowest activity at LD50 >100 μg/mL while compounds 2.1 and 2.3 were not tested. The structures of the isolated compounds were elucidated based on spectroscopic and spectrometric analyses. The results showed that some of the isolated compounds are potential anticancer agents, hence the need to extend a test to other panel of cancer cell lines.
Description
Available in print form, East Africana Collection, Dr. Wilbert Chagula Library, Class mark (THS EAF QK865.K65)
Keywords
Botanical chemistry, Erythrina, Tuberculosis, Cancer
Citation
Kombo, M. K (2014) A new pterocarpan and other constituents from erythrina schliebenii and cleistochlamys kirkii: anti-tuberculosis and anti-cancer evaluation, Master dissertation, University of Dar es Salaam. Dar es Salaam.