Characterization of diarrhoeagenic bacteria and protozoa isolated from stool of under-five children in Dar es salaam

dc.contributor.authorNgoso, Benjamin Enos
dc.date.accessioned2020-04-28T10:41:31Z
dc.date.available2020-04-28T10:41:31Z
dc.date.issued2013
dc.descriptionAvailable in printed form, East Africana Collection, Dr. Wilbert Chagula Library, Class mark (THS EAF RJ456.D5T34N456)en_US
dc.description.abstractDiarrhea is the passage of loose watery stool more than three times in twenty-four hours (WHO, 2007). It is caused by a variety of pathogens including bacteria, fungi, viruses and protozoa. The global deaths due to diarrhea have steadily declined from 4.6 million in the 1980s (Snyder and Merson, 1982) to 3.3 million in the 1990s (Bern et a I., 1992), and presently is around 2.5 million (Kosek, Bern, and Guerrant, 2003). This study was carried out to determine bacteria and parasitic protozoa that commonly cause diarrhea diseases among the under-five in Dar es Salaam The study was carried out at three district hospitals in Dar es Salaam city namely, Amana, Temeke and Mwananyamala hospitals between June 2009 and February 2010. This included children under the age of five years admitted due to acute or chronic diarrhea. A total of 360 stool samples were analyzed, of which 180 samples were from diarrhea cases and 180 samples from normal control cases. About 60% (108) of the patients were aged less than 3 years and 54.6% were males. Diarrhoeagenic bacteria were isolated and identified using conventional stool cultures then were characterized by mPCR. Parasitic protozoa were investigated by standard microscopy. Furthermore, parasitic protozoa were characterized by mPCR. Enteric pathogens were detected in 67.7% of the cases and in 20% of the controls. The pathogens most strongly associated with diarrhea disease were diarrhoeagenic Escherichia coli (21.6% of cases, 6% of controls), Shigella spp. (16.1% of cases, 5% of controls),en_US
dc.identifier.citationNgoso, B. E (2013) Characterization of diarrhoeagenic bacteria and protozoa isolated from stool of under-five children in Dar es salaam, Master dissertation, University of Dar es Salaam. Dar es Salaam.en_US
dc.identifier.urihttp://41.86.178.5:8080/xmlui/handle/123456789/10344
dc.language.isoenen_US
dc.publisherUniversity of Dar es Salaamen_US
dc.subjectDiarrheaen_US
dc.subjectBacteria diseases in childrenen_US
dc.subjectKinondoni municipalityen_US
dc.subjectDar es Salaamen_US
dc.subjectTanzaniaen_US
dc.titleCharacterization of diarrhoeagenic bacteria and protozoa isolated from stool of under-five children in Dar es salaamen_US
dc.typeThesisen_US

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