Bioconversion of coffee pulp waste and sugarcane molasses into bioethanol using yeast indigenous to coffee environment of Tanzania
| dc.contributor.author | Hamadi, Said | |
| dc.date.accessioned | 2019-12-09T13:32:45Z | |
| dc.date.accessioned | 2020-01-07T15:45:56Z | |
| dc.date.available | 2019-12-09T13:32:45Z | |
| dc.date.available | 2020-01-07T15:45:56Z | |
| dc.date.issued | 2013 | |
| dc.description | Available in print form, East Africana Collection, Dr. Wilbert Chagula Library, Class Mark (THS EAF TP360 H35) | en_US |
| dc.description.abstract | Coffee processing wastes and sugarcane molasses are among the most abundant agro wastes in Tanzania usually disposed without much use, therefore pose environmental pollution. The current study therefore aimed at developing an alcoholic fermentation process using coffee pulp waste and sugarcane molasses as substrate and using yeast indigenous to coffee environments. This study isolated a total of 21 yeasts in coffee environments of Hai and Mbinga districts in Tanzania and initially screened for ability to ferment glucose. Nine isolates were found to be fermentative and were identified using molecular method through Polymerase chain reaction (PCR) and phylogenetic analysis of D1/D2 domain of 26S rDNA. Upon evaluation of PDA1, PDA3, PDA4MB, PDA5MB, MM1-5, M2, M4 and M5 isolates on coffee pulp waste (CPW) and molasses fermentation (screening), M4 (Pichia anomala) and PDA3 (Issatchenkia orientalis) were most effective and were selected for coffee pulp wastes and molasses fermentation, respectively. At optimal pH (4.5) and temperature (30 oC) with soy flour supplementation (2%), Pichia anomala produced 6.3% (v/v) ethanol after 72 h in coffee pulp waste. In molasses fermentation, Issatchenkia orientalis produced 8.54% (v/v) ethanol in 96 h at optima pH of 6.5, temperature at 30 oC and 2% of soy flour supplement. Results indicated the potential of coffee pulp waste and sugarcane molasses as substrates for bioethanol production using yeasts from the coffee processing environment. | en_US |
| dc.identifier.citation | Hamadi, S (2013) Bioconversion of coffee pulp waste and sugarcane molasses into bioethanol using yeast indigenous to coffee environment of Tanzania, Master dissertation, University of Dar es Salaam | en_US |
| dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/1780 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Dar es Salaam | en_US |
| dc.subject | Ethanol as fuel | en_US |
| dc.subject | Biomass energy | en_US |
| dc.subject | Agricultural wastes as fuel | en_US |
| dc.subject | Tanzania | en_US |
| dc.title | Bioconversion of coffee pulp waste and sugarcane molasses into bioethanol using yeast indigenous to coffee environment of Tanzania | en_US |
| dc.type | Thesis | en_US |