Immunohistochemical study of diffuse large cell hymphomas using monoclonal and polyclonal antibodies comparing routinely fixed paraffin embedded tissue and frozen sections
| dc.contributor.author | Mgaya, Edward Mathew | |
| dc.date.accessioned | 2019-08-29T20:58:36Z | |
| dc.date.accessioned | 2020-01-08T10:06:03Z | |
| dc.date.available | 2019-08-29T20:58:36Z | |
| dc.date.available | 2020-01-08T10:06:03Z | |
| dc.date.issued | 1992 | |
| dc.description | Available in print form | en_US |
| dc.description.abstract | 48 cases of diffuse large cell lymphomas (DLCLs) from Zurich were morphologically classified according to the Kiel Classification of non-Hodgkins lymphomas (NHL) and immunologically into large centrocytic 7 cases, centroblastic 23; B immunoblastic 10, peripheral T cell lymphomas (PTCLs) 6, and true histiocytic sarcoma 2 cases. 73% of the cases were of B cell, 23% of T cell and 4% of true histiocytic origin. Immunohistochemically Anti-leucocyte common antigen (LCA) PD7/26, 2B11 showed concordant immunoreactivity with 66% of DLCLs. LN2 and MB2 proved reliable B cell markers by reacting with 70% and 74% of B cell DLCLs respectively. None of the T cell lymphomas showed reactivity with these antibodies. UCHL1 proved a better T cell marker than MT1 by reacting with 72% of PTCLs against 45% of the latter. In addition 2 true histiocytic sarcomas were identified by immunophenotyping using appropriate markers. Light chain polytypic antibodies did not prove useful in detecting B cell DLCLs. Comparatively, immunophenotyping of 32 cases of DLCLs from Dar es Salaam showed 90% to be of B cell and 10% of T cell origin. Antibody PD 7/26, 2B11 showed immunoreactivity with 50% of the cases. LN1 and LN2 showed much lower percentage of immunoreactivity with B cell DLCLs than Zurich series. 2 of the 3 T cell DLCLs reacted with UCHL1. | en_US |
| dc.identifier.citation | Mgaya, E. M (1992) Immunohistochemical study of diffuse large cell hymphomas using monoclonal and polyclonal antibodies comparing routinely fixed paraffin embedded tissue and frozen sections,Masters dissertation, University of Dar es Salaam. Available at (http://41.86.178.3/internetserver3.1.2/detail.aspx?parentpriref=) | en_US |
| dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/6069 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Dar es Salaam | en_US |
| dc.subject | Lymphomas | en_US |
| dc.subject | Switzerland | en_US |
| dc.subject | Zurich | en_US |
| dc.subject | Tanzania | en_US |
| dc.subject | Dar es Salaam | en_US |
| dc.title | Immunohistochemical study of diffuse large cell hymphomas using monoclonal and polyclonal antibodies comparing routinely fixed paraffin embedded tissue and frozen sections | en_US |
| dc.type | Thesis | en_US |